Today, there is no officially accepted country with a biological weapon program. However, the rapid advances in biotechnology that have been witnessed in recent years, especially the successes in gene regulation studies, have raised such concerns. Advances in biotechnology accelerate the production of biological weapons and reduce costs. On the other hand, advances in three-dimensional (3D) printer, artificial intelligence and robot technologies increases the possibility of rapid proliferation of biological weapons that can be produced by biotechnology and on top that, strengthens the possiblity of non-state structures using these weapons for the sake of crime and terrorism. This analysis will examine the threats posed by these technologies.